大肠癌患者血清中血管内皮生长因子和一氧化氮的检测及其临床意义

Clinical significance of measuring serum vasular endothelial growth factor and NO in patients with colorectal cancer

目的 研究大肠癌患者血清中血管内皮生长因子 (VEGF)和一氧化氮 (NO)表达水平 ,探讨其在大肠癌中的临床意义。方法 分别采用酶联免疫吸附测定 (ELISA )法和分光光度法检测 5 9例大肠癌患者术前血清中VEGF和NO水平 ,并与 3 0例健康人对照。结果 大肠癌患者血清VEGF和NO表达水平均较健康人明显增高 (P <0 .0 1) ,且随着大肠癌浸润深度增加、有淋巴结转移以及Dukes分期愈晚者而显著增高 (P <0 .0 1)。血清VEGF与NO含量呈明显正相关 (r =0 .817,P <0 .0 1)。结论 VEGF和NO与大肠癌浸润和转移有关 。

Objective To assess the clinical significance of the expression levels of serum vasular endothelial growth factor (VEGF) and nitric oxide (NO) in patients with colorectal cancer.Methods Serum VEGF level was measured by enzyme linked immunosorbent assay (ELISA) and serum NO level detected by spectrophotometry in 59 colorectal cancer patients and 30 healthy individuals.Results The serum levels of VEGF and NO in colorectal cancer patients were significantly higher than those of in normal persons(P<0.01).There was a positive correlation between serum VEGF,NO levels and tumor invasion depth,lymph node metastasis and Dukes stage(P<0.01).There was also a positive correlation between serum VEGF level and serum NO level(P<0.01).Conclusion VEGF and NO correlate with the invasion and metastasis of colorectal cancer. Detection of serum VEGF and NO levels might be important index for evaluating maligment behavior of colorectal cancer. [