人垂体肿瘤转化基因1在卵巢癌组织中的表达及其意义

Human Pituitary Tumor Transforming Gene 1 Expression in Ovarian Carcinoma and Its Clinical Significance

背景与目的:人垂体肿瘤转化基因1(humanpituitarytumortransforminggene1,hPTTG1)是新近发现的一个癌基因。我们用基因表达谱芯片对晚期卵巢低分化浆液性癌进行卵巢癌相关基因的筛查,发现该基因在这种卵巢癌中明显高表达。本研究对不同临床分期、不同病理类型及不同组织学分级的上皮性卵巢癌组织hPTTG1的表达进行检测,探讨该基因在卵巢癌发生发展中的作用。方法:用非竞争性RT-PCR方法半定量检测27例卵巢癌及4例正常卵巢组织中hPTTG1mRNA的表达,用免疫组化方法检测该27例卵巢癌及18例正常卵巢组织中hPTTG1蛋白的表达。结果:在正常卵巢组织中hPTTG1mRNA有低水平的表达,而卵巢癌组织hPTTG1mRNA表达高于正常,两者之间有显著性差异(P<0.01),卵巢癌组织较正常卵巢组织表达倍增1.1～4.8,中位倍增2.4。进一步分析卵巢癌组织中hPTTG1mRNA表达水平与临床分期及病理分级的关系,未发现hPTTG1mRNA表达水平的高低与临床分期具有相关性,但发现hPTTG1mRNA倍增与肿瘤分化程度密切相关(r=0.686,P<0.05)。hPTTG1蛋白在所有卵巢癌组织中表达,而在正常卵巢组织中未检测到hPTTG1蛋白的表达。结论:hPTTG1表达在早期卵巢癌即发生改变,其表达可能与不良分化有关;hPTTG1在卵巢癌组织中高表达,可能是卵巢癌的一个分子标志。

BACKGROUND &OBJECTIVE: Human pituitary tumor transforming gene 1 ( hPTTG1) is a newly identified oncogene. We performed a large-scale screening su rvey to identify related genes expressed in advanced and poorly differentiated s erous ovarian carcinoma using cDNA microarray analysis, and found that hPTTG1 is one of highly up-regulated genes in ovarian carcinoma. This study was to exami ne hPTTG1 mRNA and protein expression in various epithelial ovarian carcinoma,an alyze the relationship of its expression level with FIGO stage and histological grade, and explore the functional role of hPTTG1 in ovarian carcinoma pathogenes is. METHODS: HPTTG1 mRNA expression in 27 cases of epithelial human ovarian carc inoma, and 4 cases of normal ovary was assessed with non-competitive reverse tr anscription polymerase chain reaction (RT-PCR), and hPTTG1 protein expression i n these 27 cases of human ovarian carcinoma, and 18 cases of normal ovary was ex amined by immunohistochemistry. RESULTS: HPTTG1 mRNA expression level in normal ovary was low, while in ovarian carcinoma was significantly higher than that in normal counterparts (P < 0.01). Fold increase of ovarian cancer tissues to norma l ovary tissues is 1.1-4.8 (median 2.4).HPTTG1 mRNA high expression level was r elated to poor tumor differentiation (r = 0.686, P < 0.05), but the correlation with FIGO stage was not detected. HPTTG1 protein was expressed in all cases of o varian carcinoma, but not in normal ovaries. CONCLUSIONS: Increased hPTTG1 expre ssion may be an important factor involved in early tumorogenesis, and may be ass ociated with poor tumor differentiation. HPTTG1 could be a potential molecular m arker of epithelial ovarian carcinoma.