紫叶酢浆草鳞茎离体培养及快速繁殖研究

Research on Rapid in Vitro Propagation of Bulb of Oxalis violalea

采用紫叶酢浆草(Oxalisviolalea)鳞茎作外植体源,进行离体培养及快速繁殖研究。结果表明:MS+6-BA2 0mg/L+NAA0 5mg/L诱导丛生芽发生,效果较好;丛生芽增殖用MS+6-BA3 0mg/L+NAA0 5mg/L每20d转接1次增殖3～4倍。1/2MS+NAA2 5mg/L+IBA1 0mg/L有利于壮苗和生根。从生根诱导到试管苗出瓶需20d,生根率100%.从丛芽增殖至生根出苗,整个培养周期只需40d.较已有报道的用叶片、叶柄组织培养的提前15～10d,且繁殖速度极快,试管苗生长健壮,炼苗成活率达95%.

Rapid in Vitro propagation of bulbs of Oxalis violalea was studied in this paper. The results showed as follows: the MS medium supplemented with 2.0?mg/L 6-BA+0.5?mg/L NAA was suitable for inducing axillary buds. MS +3.0?mg/L 6-BA+0.5?mg/L NAA was used for proliferation and the proliferation rate could reach 3～4 times in 20 day. 1/2?MS+2.0?mg/L NAA+1.0?mg/L IBA was suitable for rooting and getting strong plants. 20 d were needed from root induction to tube plant and the rooting rate could reach 100%. One cycle which was from proliferation to forming roots only needed 40?d. Bulb culture could be shortened for 10～15?d in contrast to culture of leafstalk and leaf which was reported before. The tube plants was very strong and the survival rate could reach to 95%.