阿司匹林、舒林酸对3AO细胞环氧合酶-2、血管生长因子、层粘蛋白受体、CD_(44)V_6表达的影响

Effects of aspirin and sulindac on expression of cyclooxygenase-2, vascular endothelial growth factor, laminin receptor, and CD_(44)V_6 in 3AO cells

目的 :研究阿司匹林、舒林酸对 3AO细胞环氧合酶 2 (COX 2 )、血管生长因子 (VEGF)、CD44V6及层粘蛋白受体 (LN R)表达的影响。方法 :采用MTT方法观察阿司匹林、舒林酸在贴壁前后加入培养液中对 3AO细胞生长的影响。采用免疫细胞化学的方法研究两种药物对 3AO细胞COX 2、VEGF、CD44V6、LN R的影响。结果 :MTT实验显示阿司匹林、舒林酸不论在贴壁前还是后加入均可抑制 3AO卵巢癌细胞的生长 ,加药后调节pH可减弱生长抑制作用但仍然可明显抑制肿瘤细胞的生长。阿司匹林和舒林酸对COX 2、VEGF的表达也有抑制作用 ,但对CD44V6及LN R的表达无明显影响。结论 :抑制COX 2的表达可以下调VEGF的表达 ,从而抑制肿瘤的血管生成。阿司匹林和舒林酸可通过抑制COX 2及VEGF的表达而抑制肿瘤的生长。

AIM: To investigate the effects of aspirin and sulindac on the expression of cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), CD 44V 6 and laminin receptor (LN-R) in 3AO cells. METHODS: The growth inhibition effects of aspirin and sulindac in 3AO cells were tested by MTT assay. The expression of COX-2, VEGF, LN-R and CD 44V 6 were evaluated by immunocytochemistry. RESULTS: Aspirin and sulindac significantly inhibited the growth of 3AO cells whether added before or after cells adhered. When the pH was adjusted with sodium bicarbonate, the inhibition had a little decrease. Aspirin and sulindac also inhibited the expression of COX-2 and VEGF. But there were no effect on the expression of LN-R and CD 44V 6. CONCLUSION: Inhibited expression of COX-2 may down-regulate the expression of VEGF, and inhibit the angiogenesis of tumor. Aspirin and sulindac may exert their chemopreventive and anticancer effects by inhibiting the expressions of COX-2 and VEGF.