肉碱和长链脂肪醇对小鼠大脑乙酰胆碱的影响

Effect of carnitine and policosanol on acetylcholine concentration of cerebrum in BALB/C mice

目的 探讨肉碱和长链脂肪醇对小鼠大脑乙酰胆碱 (Ach)含量的影响。方法 用析因实验设计方法将BALB/C成年雄性小鼠随机分为标准对照、高脂对照、肉碱、长链脂肪醇、肉碱和长链脂肪醇合用共 5组 ,标准对照组饲标准饲料 ,其它各组饲含 2 0 %油脂的饲料 ,肉碱 [剂量 50mg/ (kg·鼠重 ) ]和长链脂肪醇 [剂量 50mg/ (kg·鼠重 ) ]以0 0 9%的剂量加到饲料中连续喂养 3 0d后 ,称体重、脑重 ,计算脑体比 ,用碱性羟胺比色法测大脑组织中Ach含量。结果 肉碱和长链脂肪醇对成鼠体重、脑重、脑体比无显著影响 (P >0 0 5) ;高脂饲料组小鼠脑内Ach含量较对照组明显降低 (P <0 0 5) ,长链脂肪醇可引起高脂动物脑内Ach含量升高 (P <0 0 5) ,但与正常饲料组无明显差异 (P >0 0 5) ;肉碱引起脑内Ach含量明显增多 (P <0 0 5) ;但两者联合作用时有交互作用 (P <0 0 1) ,且脑内Ach含量约等于者单独作用的差值。结论 肉碱、长链脂肪醇能分别促进小鼠大脑Ach含量的增加 。

ObjectiveTo study the effect of carnitine and policosano l on acetylcholine concentration of cerebrum in mice.MethodsThe adult BALB/C mice wre randomly divided into f ive groups,normal control group,high-fat-feeding group,carnitine group,policosan ol group,carnitine and policosanol group.The mice in the normal control group w ere fed standard forage.The mice in other groups were fed with forage including 20% fat,saturated grease 50% and unsaturated oil 50%.The carnitine dose was 50 ?mg/kg by mice weight.The policosanol dose was 50?mg/kg by mice weight.The car nitine and policosanol dose was 50?mg/kg carnitine and 50?mg/kg policosanol.Th e experimental agents were included in the forage as the content of 0.09%.All th e mice were fed orally for 30?d.The body weight and the brain weight of the mic e were weighted,the ratio of brain weight and body weight were calculated.The Ac etylcholine concentration of the cerebrum were measured by the alkalescence hydr oxylamine colorimetry.ResultsThe body weight,brain weight and the ratio of bra in weight and body weight of the mice were unsignificantly different between the five groups( P >0.05).High-fat-feeding significantly decreased the acetylchol ine content of corebrum in mice( P <0.05).Policosanol 50?mg/kg significantly increased the acetylcholine content of cerebrum in mice( P <0.05),but the incr ement resulted by the policosanol was no more than that in the standard group( P >0.05).Carnitine significantly increased the acetylcholine content of cereb rum in the mice( P <0.05),and the increment of the cerebrum acetylcholine cont ent were the most among the groups.There were interaction between carnitine resu lted by the carnitine combining policosanol was about to the subtraction of the increment resulted by the carnitine and the increment by policosanol,which was a ntagonistic action between the carnitine and the policosanol.ConclusionCarnitine and policosanol respectively increas ed the cerebrum acetylcholine content,but the combining action of carnitine and policosanol was antagonistic action.