汞致急性肾损伤量效关系的探讨

Study on dose-effect relationship between exposure to mercury and acute nephrotoxicity

目的 以尿N -乙酰 -β -D氨基葡萄糖苷酶 (NAG)活性、蛋白和尿素氮 (BUN )含量为指标 ,研究不同染汞剂量与肾损伤程度的关系 ,并探讨汞暴露与肝、肾、尿汞含量之间的相关性。方法 Wistar大鼠 2 4只 ,随机分成 4组。第 1组为阴性对照组 ,皮下注射 0 9%生理盐水 ;其余 3组分别皮下注射 0 75,1 5,2 5mg/kg体重的氯化汞溶液。注射后 12h将大鼠移入代谢笼 ,收集 12h尿样。注射后 48h采取血样和肝肾组织 ,测定尿NAG、尿蛋白和尿汞含量、血浆BUN量和肝肾汞含量。结果 不同剂量染汞组肝、肾、尿中汞含量有显著性差异 ;2 5mg/kg体重染汞组肝汞含量显著高于其他组 ;1 5mg/kg体重染汞组尿汞含量即出现显著升高 ;不同染汞剂量组间肾汞含量均有显著性差异。汞暴露量与肝、肾、尿中汞负荷呈显著正相关。染汞剂量升高 ,肾损伤加重 ,至 2 5mg/kg体重染汞组尿NAG、尿蛋白、BUN均显著高于其他染毒组。结论 染汞剂量与汞体负荷存在剂量反应关系 ;染汞剂量增加 ,肾损伤加重 ;尿汞可作为急性汞中毒的早期判定指标。

ObjectiveUsing urinary NAG,urinary protein and BUN as in dices to study whether there was dose-effect relationships between the exposed d ose of mercury chloride and the acute nephrotoxicity,and to observe the relation ship between the exposed dose of mercury chloride and level of mercury in body. MethodsTwenty-four Wistar rats were divided into four gr oups randomly.The control group was given a subcutaneous injection of 0.9% salin e.The other groups were given mercuric chloride by subcutaneous injection of 0.7 5 ,1.5 and 2.5mg/(kg·weight).12-hour Urine was collected after being injected 1 2 hours later and the blood,kidney and liver were gotten after being injected 48 hours later.Mercury concentrations in liver,kidney and urine,the activity of urinary NAG and the level of urinary protein and BUN were determined. ResultsThe variation of mercury concentrations in liver, kidney and urine among the four groups were significant.There was significantly increase of mercury concentration in liver when the dose of mercuric chloride re ached 2.5?mg/(kg·weight) and in urine when 1.5?mg/(kg·weight).The most signi ficance was observed in kidney.The positive correlation was obtained between the do se of mercury chloride exposed and the level of mercury in body.The more mercuri c chloride was exposed,the worse acute nephrotoxicity was.The significant increa se of urinary NAG,urinary protein and BUN were observed when the rats were injec ted 2 5?mg/(kg·weight) mercuric chloride.ConclusionThere was dose-effect relationship between the exposed levels of mercuric chloride and the mercury concentration in body.The a cute nephrotoxicity was worse with the dose of mercuric chloride increasing.The mercury concentration in urine can be used for the early determinant indexes of acute nephrotoxicity.