耐甲氧西林金黄色葡萄球菌及肠毒素的检测

Detetion of methicllin staphylococcus aureus and survey on staphylococcus aureus enterotoxin

目的 评价乳胶结合实验检测耐甲氧西林金黄色葡萄球菌 (MRSA)的方法并检测MRSA的肠毒素。方法 收集 130株金黄色葡萄球菌临床分离株 ,通过药敏试验将其分为耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌 (MSSA) ,应用乳胶结合试验和基因扩增分别检测青霉素结合蛋白 2a(PBP2a)和MecA基因 ;用酶联免疫吸附试验 (ELISA)检测耐甲氧西林金黄色葡萄球菌肠毒素。结果 6 5株MecA基因扩增阳性菌株中的 6 2株PBP2a检测阳性 ,6 3株MSSA中 ,MecA基因扩增及PBP2a检测全部为阴性。MRSA产肠毒素为 6 7株。MSSA肠毒素为 19株。结论 乳胶结合试验是一种简便、快速、准确检测MRSA的方法 。

Objective To evaluate of Latex fixation tests for detection of methicillin resistant staphylococcus aureus and to detect of staphylococcus aureus enterotoxin.Methods 130 staphylococcus aureus isolates extracted clinically were classified into methicllin resistant staphylococcus aureus (MRSA) and methicllin sensitive staphylococcus aureus (MSSA) based on the antibiotics sensitive test,A latex fixation tests to detect penicillin-bindingprotein 2a,was compared with the MecA PCR for the detection of methicllin resistance in staphylococcus aureus and the detection of staphylococcus aureus enterotoxin by Enzyme linked immunosorbent assay test.Results Of the 65 MecA gene positive isolates,62 were positive by the latex fixation,and all of the 63MecA gene negative strains were negative by latex fixation tests.MRSA with enterotoxin were 67 strains,by comparison,MSSA were 19 strains.Conclusion Latex fixation test was simple,fast and accurate for detection of MRSA and great emphasis should be on the detection of staphylococcus aureus enterotoxin in clinical laboratory.