结直肠癌p16蛋白的检测研究

Detection of p16 protein of colorectal cancer

目的 探讨 p16蛋白在结直肠癌的表达情况及在结直肠癌临床应用中的意义。 方法 免疫组织化学SP法检测结直肠 p16蛋白。结果 37例结直肠癌标本中 p16蛋白的阳性率为 75 7% ,在高、中和低分化癌中的阳性率分别为 87%和 17% ;在直肠癌中 p16蛋白的阳性率为 6 8%低于结肠癌的阳性率为 10 0 % ,并存在显著性差异 ;在Dukes分期中A、B期与C、D期 p16蛋白的阳性率分别为 81%和 6 9% ,在淋巴结未转移的结直肠癌中 p16蛋白的阳性率高于淋巴结转移的阳性率。结论 p16蛋白与结直肠癌的发生发展有关 ,与结直肠癌的组织学分级有关 ,而与Dukes分期以及淋巴结是否转移存在一定关系。

Objective To detect the expression of p16 protein in colorectal cancer and its significance in clinical application.Methods Immunohistochemistry S-P was used to detect the p16 protein of colorectal cancer.Results The positive rate of p16 protein was 75.7% in 37 cases,In high,mediate and low differentiated colorectal cancers was 87%,17%,respectively,the positive rate (100%) in colonic tumor was higher than the recta's tumor,and then between them exist the evidence differences.In Dukes stage,the AB and CD positive rate was 81% and 69%,respectively.The lymph node without metastasis was higher(positive rate 81%)than the lymph node with metastasis's positive rate 69%.Conclusion The p16 protein was associated with the occurrence and development of colorectal cancer,and the same as the histology classification,Dukes stages and whether metastasis of lymph node.The p16 protein can be considered the indication of nature cause of disease and prognosis of colorectal cancers in the future clinical applications.