亚慢性镉暴露对雄性大鼠生殖毒性的研究

Study on reproductive toxicity of subchronic cadmium exposure in male rats

目的 探讨亚慢性镉暴露对雄性大鼠生殖系统的毒作用。方法 2 4只清洁级SD系雄性大鼠 ,分为对照组、低、中及高剂量染镉组。分别给予 0 ,0 1,0 2 ,0 4mgCd2 + /kg体重皮下注射染毒 5周。实验结束后剖杀动物取睾丸、附睾组织待检。结果 (1)高剂量组睾丸脏器系数高于正常对照组 (P <0 0 5 ) ;各染镉组精子数、活动率均低于对照组 (P <0 0 5 ) ,而畸形率明显上升 (P <0 0 5 ) ;(2 )中、高剂量组睾丸组织血色素水平与丙二醛 (MDA)含量显著性升高 (P <0 0 5 ) ,而金属硫蛋白 (MT)含量只有高剂量组高于对照组 (P <0 0 5 )。结论 亚慢性镉暴露对大鼠睾丸有明显的毒性作用 ,其机制与镉致睾丸脂质过氧化、睾丸MT不易被诱导或保护作用较低有关。

Objective To explore the effects of subchronic cadmium exposure on male sexual systems.Methods 24 male rats of SD-strain were divided into four groups.0,0.1,0.2 and 0.4?mg Cd 2+ /(kg·bw) were injected subcutaneously in different groups for 5 weeks.After the exposure,the rats were sacrificed and the testis,epididymis were separated to detect.Results The testis tissue coefficient increased in the high dose group;sperm concentration,viability decreased,while sperm malformation rate increased in every dose groups.The hemoglobin,MDA contents increased significantly in the middle and high dose groups,but the content of MT increased only in high dose group.Conclusion Subchronic cadmium exposure can induce male sexual glands injury,especially to the testis.The mechanism may relate to peroxiodative injury,the lack of protection effect of MT.