CD44和CD54在裸小鼠大肠癌肝转移模型中的表达

Experimental Study on the Expression of CD44 and CD54 in Liver Metastasis of Colorectal Carcinoma

目的 研究CD4 4、CD5 4在大肠癌肝转移动物模型中的表达 ,寻找一个稳定、有效的早期诊断大肠癌肝转移的生物学指标。方法 取 4～ 6周龄裸小鼠 4 2只 ,其中 36只用来建立大肠癌肝转移动物模型 ,其余 6只为正常对照组。 36只动物模型按时相分成 6组 ,分别于第 10d、15d、2 0d、2 5d、30d、濒临死亡时摘眼放血处死 ,获得血清标本 ,用ELISA法测定裸鼠血清中CD4 4、CD5 4和CEA的含量 ,并观察、记录肝脏转移肿瘤灶的大小和数量。 6只正常裸鼠与实验组同笼饲养并作相应检测 ,与实验组进行对照比较。结果 对不同时相大肠癌肝转移动物模型血清中CD4 4、CD5 4和CEA的动态测定发现 :随着肿瘤的发生、发展和转移 ,动物血清中CD4 4和CD5 4的表达逐步增高 ,与肿瘤生长时间、肿瘤大小及转移程度成正比 (P <0 .0 5 )。结论 CD4 4和CD5 4可以作为早期反映大肠癌肝转移的肿瘤标志物。

Objective To study the significance of CD44 and CD54 expression in animal models with liver metastasis of colorectal cancer and try to find a stable biological marker for the early diagnosis of liver metastasis of colorectal cancer. Methods Fourty two nude mice of 4-6wks were adopted in the study. Thirty-six were used to establish animal models with liver metastasis of colorectal cancer, the rest 6 mice were used as the controls. 36 mice of the experimental group were divided into 6 subgroups, each containing 6 mice, in which low grade colorectal adenocarcinoma cells were injected in the subcapsule of the spleen.On the day 10th,15th,20th,30th day and at the time of the brink of death, animds in each subgroup were sacrificed by eyeball removal and blood letting. Serum CD44, CD54 and CEA levels of the nude mice were measured by ELISA and the sizes and number of liver metastatic loci were observed and noted. The 6 mice for control were raised in the same cages and were killed on the 30th day, and were compared with that in the experimental group. Results Through the measurement at different phrases, we found with the development progression and metastasis of cancer,CD44 and CD54 levels in the animal serum tend to increase gradually and were correlated positively with the growth time,size,and extent metastasis of the cancer. Conclusion CD44 and CD54 may be early tumor markers for liver metastasis of colorectal cancer.