异种大动物移植模型中内皮细胞诱导的自然杀伤细胞杀伤活性变化的体外研究

Study on the Cytotoxicity of Natural Killer Cells Induced by Endothelial Cells in Vitro in the Model of Xenotransplantation

目的 探讨体外条件下猪血管内皮细胞诱导的猴NK细胞杀伤活性的改变及其与细胞因子含量变化的相互关系。方法 将中国猕猴的NK细胞与多聚甲醛固定后的猪血管内皮细胞按不同比例进行体外混合培养 ,检测NK细胞在以不同比例接触靶细胞后的杀伤活性变化以及培养液上清液中的IFN γ及TNF α含量。结果 猴NK细胞与猪血管内皮细胞混合培养后 ,在一定范围内其杀伤活性随混合比例的升高而逐步升高 ,同时其IFN γ及TNF α分泌量也不断增加。结论 NK细胞与异种靶细胞接触后 。

Objective To explore the change of the cytotoxicity of natural killer cells induced by vascular endothelial cells in vitro and the relationship between this change and the variety of cytokine level. Methods After fixed by paraformaldehyde, vascular endothelial cells from pigs were co-cultured in vitro with natural killer cells from Chinese monkeys at different ratios. The change of the cytotoxicity of natural killer cells occurring after this contact and the content of IFN-γ and TNF-α in the supernatants were detected. Results The cytotoxicity of natural killer cells improved gradually in accordance with the co-culture ratio after co-cultured with fixed vascular endothelial cells. The secretion of IFN-γ and TNF-αalso improved gradually. Conclusion After contact with xeno-target cells, the cytotoxicity of natural killer cells and the secretion of cytokines are related to the ratio of effecter cells and target cells.