PDTC对重症急性胰腺炎的抑制作用

Pyrrolidine Dithiocarbamate (PDTC) Ameliorates Severe Acute Pancreatitis

目的 探讨抗氧化剂四氢化吡咯二硫代氨基甲酸脂 (PDTC)对重症急性胰腺炎 (SAP)的抑制作用。方法 Wistar大鼠 72只 ,随机分为 :假手术组 (shamoperation ,SO)、SAP组和SAP +PDTC组。术后分四个时段 ( 1、3、6、12h)分批进行下腔静脉采血后处死 ,取胰腺组织作病理切片。采用双抗体夹心ABC ELISA法测定血浆TNF α、IL 6。结果 SAP组血浆细胞因子TNF α和IL 6浓度较SO组增高 (P <0 .0 5 ) ,其中TNF α于术后 1h达到峰值 ( 871.6± 5 .9pg/ml) ,与SO组 ( 2 5 .2± 1.2 pg/ml)差异显著 (P <0 .0 0 1)。而IL - 6于术后 3h达到峰值( 5 9.2± 4 .1pg/m) ,与SO组 ( 2 5 .2± 1.2 pg/ml)差异显著 (P <0 .0 5 )。SAP +PDTC组的血浆TNF α和IL 6浓度较SAP组明显下降 (P <0 .0 5 ) ,胰腺局部炎症反应减轻。结论 细胞因子TNF α和IL 6是SAP炎症因子网络中重要的组成部分 ,可以作为判断AP严重程度和预后的预测指标 ;PDTC抑制NF κB的活化 ,不仅抑制了SAP时SIRS的过程 。

Objective To investigate the roles of antioxidant, pyrrolidine dithiocarbamate (PDTC) in severe acute pancreatitis (SAP). Methods Seventy two Wistar rats were randomly divided into sham-operation(SO) group, SAP group, and SAP previously treated with PDTC group. SAP was induced in rats by injection of 5% sodium taurocholate into biliopancreatic duct. Plasma TNF-α and IL-6 were determined at 1, 3, 6 and 12 hour after operations. Results The plasma cytokine TNF-α and IL-6 in SAP group increased obviously compared with that in SO group ( P <0.05). The TNF-α reached its peak at 1 h while IL-6 did at 3 h postoperatively. The treatment with PDTC, markedly decreased the plasma TNF-α and IL-6 level compared with that in SAP group ( P <0.05), and also ameliorated the histologic injury of pancreas. Conclusions PDTC, an inhibitor of NF-κB activation, might alleviate the inflammatory response in SAP by suppressing cytokine gene expression.