6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达

Cloning,sequencing and expression of glucitol-6-phosphate dehydrogenase(gutD) gene

本研究根据6 磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物,以假单胞杆菌(PseudonmonasXM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析.将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Es cherichiacoli)JM101后检测其蛋白表达及菌株生长耐盐性.

A glucitol-6-phosphate dehydrogenase (gutD) was cloned viaPCR method using Pseudomonas genomic DNA as template. The gutD gene was sequenced and expressed in pBV220 vector. The transformant was tested for its salty tolerance.