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南海沉积物中有孔虫分子生物学鉴定的初步研究 被引量：2

Preliminary study on SSU rDNA of foraminifera from South China Sea sediment
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摘要 本文以南海深海表层沉积物中的有孔虫样品为研究对象,提取了样品的总DNA片段,利用特异性DNA探针,进行有孔虫的SSUrDNA扩增,对获得的PCR产物进行克隆,测序及分析,证实确为潜在的有孔虫DNA.进化树分析揭示了研究样品中可能存在的有孔虫种类。 The foraminifera sample from deep-sea surface sediment in theSouth China Sea was used in the study. The total DNA was extracted and the foraminiferaSSU rDAN was amplified by specific DNA probe. The PCR products obtained werecloned, sequenced and analyzed, and were proved to be potential foraminifera DNA. The phylogenetic tree analysis reveald the potential foraminifera species inthe studied sample. The establishment of the method should provide a new technological measure for identification of foraminifera tests.
作者 徐美香 赵泉鸿 肖湘
机构地区 国家海洋局海洋生物遗传资源重点实验室 上海同济大学海洋地质教育部重点实验室
出处 《台湾海峡》 CAS CSCD 2004年第3期354-359,共6页 Journal of Oceanography In Taiwan Strait
基金 国家海洋局海洋生物工程重点实验室研究基金
关键词 海洋浮游动物 分子生物学:有孔虫 DNA提取 SSU rDNA 进化树 molecular biology foraminfera DNA extraction SSU rDNA phylogenetic tree South China Sea
分类号 Q75 [生物学—分子生物学] P736.21 [天文地球—海洋地质]
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参考文献7

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  • 2Pawlowski J, Bolivar I, Guiard-Maffia J, et al. Phylogenetic position of foraminifera inferred from LSU rRNA gene sequence[J]. Molecular Biology and Evolution,1994,11(6):929～938.
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同被引文献38

  • 1王飞飞,赵全民,丁旋,刘健.渤海东北海域有孔虫埋葬群特点与沉积环境的关系[J].海洋地质与第四纪地质,2009,29(2):9-14. 被引量：13
  • 2李元芳,何希贤.渤海湾北岸表层沉积中的有孔虫群及其生态环境[J].地理研究,1983,2(1):65-73. 被引量：9
  • 3李保华,Kemal Topac Ertan,Christoph Hemleben.有孔虫分子生物学研究进展[J].自然科学进展,2005,15(5):534-538. 被引量：3
  • 4李保华,李铁刚,Kermal Topac Ertan,Christoph Hemleben,李春香,曹奇原,杨群.西北太平洋浮游有孔虫的SSU rDNA序列及其古海洋学意义[J].自然科学进展,2007,17(3):391-395. 被引量：5
  • 5成鑫荣,1987.长江口表层沉积物中活有孔虫分布的初步研究.海洋地质与第四纪地质,7(1):73—79.
  • 6汪品先,章纪军,闵秋宝,1980.东海表层沉积物中有孔虫的分布.见:海洋微体古生物论文集.北京:海洋出版社,20-38.
  • 7Boucheta V M P, Alvea E, Rygg B et al,2012. Benthicforaminifera provide a promising tool for ecological qualityassessment of marine waters. Ecological Indicators, 23:66-75.
  • 8Eichler P P B, Eichler B B, Gupta B S al, 2012. Foraminiferaas indicators of marine pollutant contamination on the innercontinental shelf of southern Brazil. Marine PollutionBulletin, 64(1): 22-30.
  • 9Hayward B W, Holzmann M, Grenfell H K et al, 2004.Morphological distinction of molecular types inAmmonia-towa.rds a taxonomic revision of the world’s mostcommonly misidentifled foraminifera. MarineMicropaleontology, 50(3-^): 237-271.
  • 10Holzmann M, Pawlowski J, 1996. Preservation of foraminiferafor DNA extraction and PCR amplification. Journal ofForaminiferal Research, 26(3): 264-267.

二级引证文献15

台湾海峡
2004年 第3期

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