水生细菌生物量的估算方法

Methods to Assess Biomass of Aquatic Bacteria

细菌的生物量是海洋微生物研究的关键参数之一。目前 ,细菌生物量估算方法主要根据其体积的大小进行换算 ,但换算的方式也不尽一致。对细菌体积和细菌碳含量主要的测定方法、体积与生物量之间换算系数、换算模式进行介绍 ,评述。认为流式细胞术是测定细菌体积较为合适的方法 ,X 射线微量分析法是测定单个细菌生物量 (碳含量 )最合适的方法 。

Bacterial biomass is considered as one of the central parameters in the research on marine ecosystems. Now, the methods to convert bacterial biomass could depend on bacterial volume, but there is some difference among the different conversion models. Not only the measurements of bacterial volume and bacterial carbon concentration, the conversion factor between the volume and bacterial biomass, but also the conversion model will be discussed in this paper. Conclusions can be reached that flow cytometry is available to assess bacterial volume and X ray microanalysis is the best method to measure single bacterial biomass and allometric model is widely used in conversion of biomass.