摘要
目的从DNA分子水平上分析新疆药用桑树资源9个栽培群体的遗传关系。方法从100个随机引物中筛选出10个引物,用这10个引物做RAPD扩增,应用NTSYS软件对扩增结果进行聚类分析。结果共扩增出108个位点,其中多态性位点91个,多态比例为84.26%,各群体间存在较明显的遗传分化。另外,获得药桑的8个特异位点。结论RAPD分析结果与新疆药用桑树资源植物的遗传关系的传统划分是基本一致的。
Object To study the inherited relationships among nine medicinal mulberry populations cultivated in different regions of Xinjiang at DNA level Methods DNA of all populations was amplified by ten random primers from 100 ramdom primers, the polymorphic bands of RAPD were counted And the results of populations were analyzed by clustering analysis NTSYS PC software Results A total of 108 bands was obtained, 91 of total bands (84 26%) was polymorphic The results indicated that there were apparent and abundant genetic variations in Morus L of Xinjiang Besides, eight specific bands of M nigra were obtained Conclusion The genetic relationship among medicinal resources plants of mulberry in Xinjiang indicats by the RAPD analysis to be consistent with their traditional classifications in the main
出处
《中草药》
CAS
CSCD
北大核心
2004年第9期1053-1056,共4页
Chinese Traditional and Herbal Drugs
关键词
桑树
栽培群体
RAPD
遗传多样性
mulberry
cultivated population
RAPD
genetic diversity
