柯萨奇-腺病毒受体在小鼠病毒性心肌炎心肌表达及其与心肌损害关系

Expression of coxsackievirus and adenvirus receptor and relationship between changes of its expression and myocardial damages in viral myocarditis rats

目的 探讨柯萨奇 腺病毒受体 (CAR)在病毒性心肌炎 (VM )发病中的作用。方法 30只小鼠随机分为实验组和对照组 ,所有小鼠于感染后d10处死 ,取其心脏 ,采用Westernblotting及免疫组织化学技术检测心肌细胞CAR表达水平 ,并检查心肌病理变化 ,并对CAR表达水平与心肌的病变程度行相关性分析。结果 Westernblotting显示 ,实验组存活小鼠心肌中CAR表达水平明显高于对照组 ;免疫组织化学检测显示 ,实验组小鼠心肌CAR抗原免疫反应明显增强 (t=4 .94 P <0 .0 1) ,且其CAR表达水平与心肌病变积分呈显著正相关(r =0 .91 P <0 .0 1)。

Objective To investigate the role of coxsackievirus and adenovirus receptor (CAR) in the development of viral myocarditis. Methods Thirty mice were divided randomly into experimental group and control group. The mice were killed and the hearts were removed after being infected 10 days. The expression of CAR in the myocardium were examined by Western blotting and immunohistochemistry. In addition, the levels of CAR antigen were semi- quantitatively analyzed, and the relationship between the CAR expression levels and the myocardial lesions was investigated. Results Western blotting showed that CAR expression levels were significantly higher in experimental group than those in control group. Immunohistochemistry indicated that the expression levels of CAR distinctly increased in experimental group as compared with control group, and its index of CAR antigen positive signal was signi- ficiantly positively correlated with myocardial histopathological score. Conclusion The increase of CAR expression may play an important role in development of viral myocarditis.