摘要
目的 研究惊厥发生后不同发育阶段神经元白细胞介素 1受体 (IL 1R)、Cx36基因表达。方法 无镁细胞外液短暂处理培养的发育中皮层神经元 ,用Real time定量RT PCR方法检测IL 1R、Cx36mRNA表达改变。结果 1.体外培养 6、17d神经元无镁处理后IL 1R表达呈一过性降低 ,之后 6d神经元表达恢复正常 ,而17d神经元表达增加 ,于处理后 2 4h达高峰 (P <0 .0 5 )。 2 .体外 6d的神经元无镁处理后Cx36表达渐增高 ,于处理后 2 4h达高峰。体外 17d神经元处理后 6hCx36表达与对照组比较明显降低 (P <0 .0 5 ) ,2 4h降低达峰值。结论 无镁诱导惊厥后体外 6、17d神经元IL 1R、Cx36表达不同 ,可能与惊厥造成 6。
Objective To study interleukin-1 receptor(IL-1R) and connexin(Cx36) gene expression following Mg 2+-free-induced seizures in cultured developing neuron. Methods Rat embryo cortical neurons cultured for 6 and 17 days were exposed to Mg 2+-free media to induce seizure. At different time after Mg 2+-free treatment, real-time RT-PCR was used to detect IL-1R and Cx36 mRNA expression. Results 1. IL-1R mRNA expression transiently decreased after Mg 2+-free treatment in neurons cultured for 6 and 17 days in vitro. Then the levels of IL-1R mRNA expression recovered in neurons cultured for 6 days, but IL-1R mRNA expression were increased in neurons cultured for 17 days compared with control group and the peak was at 24 hours. 2. In neurons cultured for 6 days in vitro, Cx36 mRNA expression increased after Mg 2+-free treatment compared with control group, the peak was at 24 hours. But in neurons cultured for 17 days in vitro, Cx36 mRNA expression decreased at 6 hours after Mg 2+-free treatment compared with control group, the peak was at 24 hours. Conclusions IL-1R mRNA and Cx36 mRNA expression following Mg 2+-free-induced seizures are different between the neurons cultured for 6 and 17 days in vitro. This is possibly related to the different neuron injury between 6 and 17 days in vitro following seizures.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第9期765-767,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金资助项目 (30 2 70 4 4 8)