摘要
美味猕猴桃 (AcitinidiadeliciosaC .F .liangetA .R .Ferguson)为猕猴桃科猕猴桃植物。目的 :观察美味猕猴桃根提取物对肿瘤细胞的抑制和凋亡影响。方法 :采用MTT法、集落形成法、生长曲线法和Hoechst 3 3 2 5 8荧光染色法测定美味猕猴桃根提取物对白血病细胞株L12 10和P3 88D1、宫颈癌细胞株Hela、人胃癌细胞株SGC790 1、黑色素瘤细胞株B16、神经性肿瘤细胞株NG10 8-15的生长抑制和凋亡影响。结果 :美味猕猴桃根提取物均可抑制 6种肿瘤细胞株的增殖 ,并可导致部分细胞凋亡和死亡。结论
Objective: To observe the effects of extracts from roots of Acitinidia delicilsa on the growth inhibition and apoptosis in carcinoma cell lines. Methods: The growth inhibition and apoptosis was analyzed by MTT, Hoechst 33258 fluorescence, Cell colony and cell growth curve measurement chnique in the stomach carcinoma SGC7901, Lymphocytic leukemia L1210, Lymphoid neoplasm P388D1, Tumor of cervix uteri HeLa, Melanoma B16 and mouse neuroblastoma x rat glioma hybrid NG108-15 cells lines. Results: The growth inhibition and apoptosis is induced by extracts from roots Acitinidia delicilsa in all cell lines. Conclusion: The results shown that extracts from roots Acitinidia delicilsa possesses the role of antitumors in cell culture.
出处
《中医药学刊》
2004年第9期1705-1707,共3页
Study Journal of Traditional Chinese Medicine
基金
国家自然科学基金地区联合资助项目 (3 0 160 0 95 )
国家人事部留学回国人员资助项目 (人发「2 0 0 2」99号 )
广西新世纪十百千人才工程资助项目 (2 0 0 12 0 9)
关键词
美味猕猴桃根
提取物
肿瘤细胞
Roots of Acitinidia delicilsa
Extracts
Tumor cell lines
