软肝煎药物血清对肝星状细胞免疫保护作用的研究

The Research On Serum Containing Ruanganjian Decoction's Immunoprotection Of Hepatic Stellate Cell

目的 :探讨软肝煎药物血清对肝星状细胞 (HSC)的免疫保护作用。方法 :先采用不同两种种族的动物血清培养人肝星状细胞株 (Lx -1) ,研究不同种族的血清对同一细胞的免疫损伤作用 ,然后再采用对Lx -1细胞株损伤小的血清组与软肝煎药物血清组进行比较来研究对肝星状细胞的免疫保护作用。结果 :在测定LDH活性中 ,小牛血清组与大鼠血清组比较 ,各组浓度均有非常显著性差异 (P <0 .0 1)。药物血清组与大鼠血清组比较 ,各组浓度均有非常显著性差异 (P <0 .0 1)。药物血清组 5 %与 10 %比较无显著性差异 (P >0 .0 5 ) ,浓度 5 %、10 %两组与浓度 2 0 %比较有非常显著性差异 (P <0 .0 1)。结论 :软肝煎药物血清对肝星状细胞有很好的免疫保护作用 ;

Objective:To investigate serum containing Ruanganjian decoction's immunoprotection on hepatic stellate cell.Methods:Applying two types of animal sera to culture Lx-1 which belong to a type of human's hepatic stellate cell strain at first, it aimed to evaluate how different types of animal sera have immunodamage on the same cell strain.Then adopting the group which did little of damage to the cell strain together with a drug-containing serum group,the research were made on serum containing Ruanganjian decoction's immuoprotection on hepatic stellate cell.Results:In assaying LDH activity,compared with rat serum group,calf sera groups of different concentration did less of damage to Lx-1 with more significant difference(P<0.01).Compared with rat serum,drug-containing sera groups did less of damage to Lx-1 with more significant difference(P<0.01).In drug-containting sera groups ,concentration 10% group has the same immunoprotection with no significant difference compared with concentration 5% group(P>0.05).While compared with concentration 20% group, the two groups of concentration 5% and 10% have a better immunoprotection with more significant difference(P<0.01).Conclusion:Serum containing Ruanganjian Decoction's have a better immunoprotection of hepatic stellate cell.To culture cell,the serum with belong to the same strain with the cultured cell had better be applied.