风疹病毒山东地方株的分离与鉴定

Isolation and identification of rubella virus strains from Shandong province

目的 了解山东省风疹病毒流行情况 ,进行风疹野病毒山东地方株的分离、鉴定。方法 2 0 0 1～ 2 0 0 2年 ,从我省的济宁、淄博、济南、德州市风疹爆发流行中 ,采集 5 3份具有风疹典型症状患者的咽拭子 ,经Vero ,RK-13 ,BHK-2 1,MK2 细胞分离培养 ,选出有明显的细胞病变株。结果 在Vero细胞上对HSV - 1病毒攻击呈干扰现象 ;风疹免疫荧光阳性者 ;共分离出 9株风疹病毒山东地方野毒株 (SDRV) ,代码为SDRV1、2、3、4、5、6、7、8、9。结论 细胞用于风疹病毒的分离培养 ,免疫荧光实验可作为风疹病毒鉴定。

Objective For isolation and identification of rubella virus wild strain from Shandong Province.Methods 9 wild strains of Shandong district of rubella virus were isolated from 53 throat swabs.These virus cause marked cytopathic effect (CPE) in Vero,RK-13,BHK-21,MK2 cell lines.Results 9 SDRV strains were isolated.SDRV infected Vero cell showed the interference with the invasion of HSV-1,Immunofluorescence assay were used to identify the viruses,neutralization tests were used for confirmation for newly rubella virus isolations.Conclusion Immuofluorescene can be the important method for rubella virus isolation and identification.