室内生源性多环芳烃对DNA的氧化损伤

Study on oxidative DNA damage induced by PAHs from household pollution

目的 了解室内生活污染来源 -吸烟和烹调产生的多环芳烃 (PAHs)种类与含量 ,及其对DNA的氧化损伤作用。方法 采集室内烹调油烟和环境烟草烟雾颗粒物 ,应用气相色谱 -质谱 -选择性离子监测技术 (GC -MS-SIM )定量检测 10种PAHs,并用 10种混合PAHs经气管灌注染毒大鼠 ,用液相色谱结合电化学检测技术测定肺组织DNA中产生的氧化损伤标志物 8-羟基脱氧鸟苷 (8-OHdG)。结果 PAHs广泛存在于烹调油烟冷凝物、油烟颗粒物以及环境烟草烟雾的主、侧流烟雾颗粒物之中。其标准混合物可诱导大鼠肺组织DNA氧化损伤形成 8-OHdG ,并呈现明确的剂量 -反应关系。

Objective To study the kinds and concentration of PAHs released from household pollution-environmental tobacco smoke and heated oil smoke,and the effect of inducing oxidative DNA damage.Methods Collected particles of heated oil smoke and environmental tobacco smoke were quantitatively detected by GC-MS-SIM.With the trachea instillation,8-hydroxy-2'-deoxyguanosine,the biomarker of oxidative DNA damage from the rat lung tissue induced by the 10 kinds of PAHs',was detected by HPLC-EC.Results PAHs widely existed in the condensed matters and the particle matters such as heated oil,the main and the side stream tobacco smoke.The mixture compounds of 10 kinds standard PAHs can induce oxidative DNA damage of the rat lung tissue to form 8-OHdG,and had significnat dose-response relationship.Conclusion Genotoxic and carcinogenic effects of PAHs had a path of after metabolized to yield hydroxyl radicals(·OH) to attack DNA.