登革2型病毒感染小鼠体内病毒迁移及分布

Organic distribution of dengue virus in infected mouse

目的 观察登革病毒在感染动物模型体内的迁移和分布特点 ,了解登革病毒的感染致病机制。方法 以 2型登革病毒新几内亚 (NGC)株和临床分离株病毒悬液皮下注射 ,建立登革病毒感染的小鼠模型。于感染后不同时间采集小鼠血浆、肝、脾、肾、脑等脏器组织 ,用病毒分离及RT -PCR方法检测病毒的存在。结果 2株 2型登革病毒感染小鼠后 ,在小鼠血浆、肝脏、脾脏、肾脏和大脑组织中均可检测到病毒 ,病毒在脑组织中持续存在的时间最长 ,在肝脏中滞留时间最短。临床分离株感染小鼠体内病毒持续时间较NGC株长。结论 登革病毒感染后病毒呈全身性多脏器组织分布 。

Objective To analyse the mechanism of dengue virus infection by observing the organic distribution of virus in the infected mouse.Methods Virus isolation and RT-PCR were used to detect the virus and viral RNA.The time-course detection of virus in the mouse blood plasma and liver,spleen,kidney,brain samples were observed.Results The virus existed in the mouse blood plasma and liver,spleen,kidney,brain after mouse were inoculated with dengue virus(NGC strain,clinic strain).The virus duration in the brain was longest.Further more,the virus existence in the mouse infected with clinic strain dengue virus was longer than the group of NGC strain infection.Conclusion The dengue virus was systemic distribution in the infected mouse.And the virulence of clinic strain dengue virus was harder than NGC strain.