大豆活性肽对大鼠体内高胆固醇代谢的影响

Effect of soybean peptides intake on metabolism of cholesterol in hypercholesterolemic rats

目的 探讨膳食大豆活性肽对高胆固醇模型大鼠血浆胆固醇及其有关血脂指标的影响 ,研究其可能的作用机制。方法 4周龄断乳雄性Wistar大鼠 ,经 2 8d诱发高血胆固醇以后 ,按体重和血浆总胆固醇浓度将动物均衡分为 2组 ,分别喂饲含大豆活性肽和酪蛋白的纯合成高脂饲料 5 6d ,断头处死动物。实验结果用SPSS统计软件进行统计分析 (单因素F检验 )。结果 经 2 8d诱导高脂模型后 ,实验模型大鼠的TC水平为 ( 2 0 2± 0 2 7)mmol/L (n =2 1) ,而喂饲基础饲料的阴性对照组大鼠分别为 ( 1 33± 0 2 0 )mmol/L (n =9)。高血胆固醇模型大鼠的血浆总胆固醇(TC)浓度分别是阴性对照组的 1 5 1倍。又经 5 6d喂饲含有处理因素的饲料后 ,酪蛋白组和大豆活性肽组实验动物体重分别增加了 ( 198 5和 119 3) g ;TC水平分别为 ( 6 4 1± 0 5 7)和 ( 3 0 1± 0 37)mmol/L ;TG水平分别为 ( 3 73±0 70 )和 ( 1 0 9± 0 34)mmol/L ;apoB分别为 ( 0 6 2± 0 11)和 ( 0 31± 0 0 7) g/L ;FT4分别为 ( 1 0 3± 0 2 2 )和 ( 1 76±0 30 ) pmol/L ;同酪蛋白组相比 ,大豆活性肽组动物体重及TC ,TG ,apoB含量显著降低 ,而FT4水平显著高于酪蛋白组 (P <0 0 5 )。结论 膳食中大豆活性肽的摄入可降低高血胆固醇模型大鼠TC、

Objective To invstigate the effects of dietary soybean peptides intake on the concentration of serum total cholesterol(TC) and serum lipids in hypercholesterolemic rats and to study the mechaisms.Methods Following 28 days of inducing the hypercholesterolemia model successfully,rats were fed a purified diet containing either casein or soybean peptides.After the rats were fed experimental diets for 56 days,fasting animals were killed.Results In the end of the experiment, TC was (6 41±0 57) and (3 01±0 37)mmol/L in the rats fed diets containing casein and soybean peptides,respectively.Triglyceride(TG) was (3 73±0 70) and (1 09±0 34)mmol/L,respectively.Serum apolipoprotein B(apoB) was (0 62±0 11) and (0 31±0 07)g/L,respectively.Accordingly,FT4 was (1 03±0 22) and (1 76±0 30)pmol/L,respectively.Comparing with the rats in casein group,the TC,TG and apoB in soybean peptides group were significantly low ( P <0 05).The concentration of FT4 was higher in the rats of soybean peptides group ( P <0 05).Whereas,the changes of HDL-C,apoA,FT3,TSH and insulin (INS) were not significant in these rats.Conclusion Dietary soybean peptides intake may lower TC by changing the concentrations of apoB and FT4.Further researches were needed for explaining the possible mechanism of soybean peptides intake on lowing TC.