美味猕猴桃基因组DNA的提取条件优化

Extraction of Genomic DNA from Actinidia Deliciosa

采用改良CTAB法以及不同 2 -巯基乙醇梯度和不同EDTA梯度分离基因组DNA的方法对多糖含量高、极易褐化的美味猕猴桃嫩叶基因组DNA提取条件进行了优化。结果表明 ,提取缓冲液中加入 2 0mmol/LEDTA及 4 %、5 % β 巯基乙醇的改良CTAB法和 4 0mmol/L、6 0mol/L、80mmol/L、10 0mmol/LEDTA及不同浓度的 β 巯基乙醇 (0 %～ 5 % )

To satisfy their increasing researches in molecular Biology, modified CTAB and different concentration gradient of EDTA with 2-mercaptoethanol in modified CTAB method are described in the present paper. The species contain high content of polyphenol, tannin and polysaccharide compounds that prevent t DNA from isolation. It was shown that the method of modified CTAB using 20 mmol/L EDTA extraction buffer with β-mercaptoethanol which concentration as high as 4%, 5% and using 40 mmol/L、60 mmol/L、80 mmol/L、100 mmol/L EDTA extraction buffer with different concentration gradient 13-mercaptoethanol ( 0%～5%) in the protocol was more effective.