金针菇子实体颜色基因的分子标记

Molecular marker for fruit-body color gene of Flammulina velutipes

用群体分离分析法从200个随机引物中筛选出S375,其扩增片段与金针菇子实体白色基因连锁.对S375扩增片段两端测序,以该序列为依据合成一对特征序列扩增区域(SCAR)引物,它能对带有白色基因的菌株扩增出单一片段,多聚酶链反应(PCR)产物不需电泳、加EB后可在紫外灯下直接检测.

S375 was selected from 200 random primers by bulked segregant analysis, the amplified fragment was linked with white allele of fruit-body color gene. Both ends of S375 amplified fragment were sequenced. Based on the sequences of both ends, a pair of sequence characterized amplified regions (SCAR) primers were synthesized. The SCAR primers amplified unique fragment for all strains that carry white allele of fruit-body color gene. The products of SCAR primer PCR could be detected under ultraviolet lamp directly, electrophoresis was not always necessary.