肥胖并子宫内膜癌病人腹部脂肪组织芳香化酶mRNA的表达

AROMATASE mRNA EXPRESSION IN ABDOMINAL ADIPOSE TISSUES OF OBESE PATIENTS WITH ENDOMETRIAL CANCER

①目的 了解绝经后肥胖并子宫内膜癌病人腹部脂肪组织芳香化酶mRNA的表达水平 ,并探讨其在子宫内膜癌发病中的作用及与胰岛素抵抗的关系。②方法 术中留取绝经后肥胖并子宫内膜癌、单纯肥胖妇女及正常体质量妇女腹部皮下及大网膜脂肪组织 ,进行总RNA提取 ,并应用半定量逆转录 聚合酶链反应技术检测芳香化酶mRNA的表达水平。同时测定空腹血糖及空腹真胰岛素水平。③结果 绝经后肥胖并子宫内膜癌病人脂肪组织芳香化酶mRNA的表达水平高于正常体质量妇女 (F =9.2 2、1 3.6 1 ,q =5 .39、6 .32 ,P <0 .0 1 ) ;与绝经后单纯肥胖女性比较无显著差异 (q =0 .2 6、0 .1 3,P >0 .0 5 )。绝经后肥胖并子宫内膜癌和单纯肥胖妇女脂肪组织芳香化酶mRNA的表达与空腹血清胰岛素水平呈正相关 (r =0 .6 37- 0 .737,P <0 .0 5 )。④结论 绝经后肥胖并子宫内膜癌病人腹部脂肪组织芳香化酶mRNA表达水平增高可能与子宫内膜癌发生无关 。

Objective To study the aromatase(AROM) mRNA expression in abdominal adipose tissues of postmenopausal obese women with endometrial cancer, and to explore the relationship between AROM mRNA expression and insulin resistance. Methods Subcutaneous and adipose tissues of greater omentum in postmenopausal obese women with endometrial cancer, in simple obesity women, and normal weight women were collected during abdominal surgery for RNA extraction. AROM mRNA was measured by semi quantitative reverse transcriptase and polymerase chain reaction(PCR). Fasting plasma glucose and fasting plasma insulin were also measured. Results In postmenopausal women, the AROM mRNA level in the obesity with endometrial cancer group was significantly higher than that in the control group( F=9.22,13.61;q=5.39,6.32;P <0.01), but was not significantly different from that of the simple obesity group( q=0.26,0.13; P >0.05). AROM mRNA expression in the endometrial cancer with obesity and simple obesity group was positively correlated with fasting plasma insulin( r=0.637-0.737, P <0.05). Conclusion The high expression of AROM mRNA in the abdominal adipose tissue of postmenopausal women with obesity and endometrial cancer may not correlate with the pathogenesis of endometrium cancer, but something to do with high fasting plasma insulin.