杀虫隆对末龄幼虫家蝇表皮成分及体内激素滴度的影响

EFFECTS OF TRIFLUMURON ON THE INGREDIENTS IN CUTICULAR AND THE HORMONE TITERS OF HOUSEFLY LARVAE

用不同剂量的几丁质合成抑制剂杀虫隆 (Triflumuron)饲喂家蝇 (Muscadomestica)幼虫后 ,对末龄幼虫表皮的化学成分进行定量分析 ,结果表明表皮中几丁质的含量减少了 2 2 6 %～ 4 6 3%。杀虫隆对表皮粗脂肪的合成稍有促进作用 ,但影响不显著。用高浓度 (>1mg L)杀虫隆处理家蝇幼虫后能明显提高表皮中粗蛋白的含量。用高效液相色谱法测定家蝇末龄幼虫不同时龄血淋巴中蜕皮激素和保幼激素的含量变化 ,结果表明杀虫隆能强烈抑制末龄幼虫血淋巴中蜕皮激素的滴度峰 ,但对峰期以外时间的滴度没有显著影响。杀虫隆可使末龄幼虫的保幼激素滴度在 2 4时龄前维持在高于正常幼虫的水平 ,2 4时龄后 ,对照组血淋巴中的保幼激素滴度开始上升 ,在 4 8时龄时上升到一较高值 ,而处理组却在 4

Triflumuron was used with different dosages to feed the larvae of Musca domestica .The results showed that the triflumuron inhibited the chitin synthesis in cuticular of the last instar larva by 22 6%～46 3%,and slightly stimulate the crude fat synthesis.With high concentration(>1mg/L),triflumuron increased the content of crude protein in cuticular obviously.The variations of ecdysteroid(20-E)and juvenile hormone(JH)titer in the hemolymph in different periods of the last larval instar of M.domestica were detected by high-performance liquid chromatography(HPLC).The peak value of ecdysteroid was suppressed strikingly by triflumuron.The titres of juvenile hormone(JH)remained a higher level until 24th hour age.Then the titre of JH in control group began to rise,while in the treatment group the titre declined to the lower level.