血管内皮标记物显示肺癌组织微血管密度的比较性研究及其与预后的关系

Comparison of vascular endothelial markers for microvessel density in lung cancer and their significances to survival

目的 对比性研究Ⅷ因子相关抗原 (factorⅧ relatedantigen ,FⅧ RAg)、CD31、CD34在显示肺癌组织微血管密度 (microvesseldensity,MVD)中的差异 ,以及MVD与生存期的关系。 方法 采用免疫组织化学技术 ,以FⅧ RAg、抗CD31抗体、抗CD34抗体对 5 3例手术切除、石蜡包埋肺癌组织中的微血管内皮细胞进行染色。 结果 FⅧ RAg、CD31、CD34阳性率均为 10 0 % ,MVD分别为 (2 0 .4 1± 9.6 2 )条 /HP、(2 1.84± 13.2 4 )条 /HP和 (2 1.96± 11.6 7)条/HP ,3者之间差异无统计学意义 (P >0 .0 5 ) ;血管高密度组的生存时间均明显较血管低密度组的生存时间短 (P <0 .0 5 ,P <0 .0 0 1,P <0 .0 0 0 1)。结论 提示CD34是显示肺癌组织MVD的合适的标记物 ;

Objective To evaluate the differences among three most commonly used endothelial markers for microvessel density(MVD),factor Ⅷ-related antigen(FⅧ-RAg),CD31,CD34 in lung cancer,and the relation of MVD to the survival.Methods The study consisted of 53 patients with lung carcinoma surgery.Immunohistochemistry on paraffin sections was performed with FⅧ-RAg,anti-CD31 antibody and anti-CD34 antibody to study MVD.Results The positive rates of FⅧ-RAg,CD31 and CD34 were all 100%. And their average counts of MVD were ( 20.41± 9.62),( 21.84± 13.24) and ( 21.96± 11.67)pieces/HP.MVD shown by FⅧ-RAg,CD31 and CD34 had no significant differences(P> 0.05).The survival rate of patiants with high MVD was lower than that of patiants with low MVD(P< 0.05,P< 0.001,P< 0.0001).Conclusion CD34 is a suitable marker for the immunohistochemical visualization of MVD in lung cancer.MVD is a good prognostic factor for patients with lung cancer.