β-连环素和基质溶素在大肠癌发生发展中的作用

Effect of β-catenin and matrilysin in progression of colorectal cancer

目的 :探讨 β 连环素 (β cat)、基质溶素 (MMP 7)在大肠癌发生、发展中的作用 .方法 :用免疫组织化学法检测早期大肠癌及其相应正常肠黏膜、变性隐窝灶 (ACF)、腺瘤、进展期大肠癌中β cat和MMP 7的表达 .结果 :在腺瘤、早期大肠癌、进展期大肠癌中出现 β cat细胞膜表达减少 ,细胞质和 /或细胞核阳性着色等异位表达 ,MMP 7细胞质阳性表达 .由正常肠黏膜、ACF、早期大肠癌、腺瘤至进展期大肠癌 β cat在细胞膜表达呈下降趋势 (P <0 .0 5 ) ;在细胞质或细胞核表达及MMP 7阳性率呈上升趋势 (P <0 .0 5 ) .早期大肠癌中随着肿瘤细胞分化程度的降低 ,β cat在细胞膜表达逐渐减少(P <0 .0 5 ) ,细胞质或核β cat表达及MMP 7呈阳性表达与细胞分化程度无关联 (P >0 .0 5 ) .β cat异常表达及MMP 7阳性表达与早期大肠癌浸润深度和转移相关 (P <0 .0 5 ) .早期大肠癌中MMP 7表达与β cat在细胞膜表达不相关 (P >0 .0 5 ) ,而在细胞质、细胞核表达率MMP 7阳性组高于阴性组(P <0 .0 5 ) .结论 :β cat,MMP 7参与大肠癌的发生、浸润和转移 .

AIM: To investigate the correlation of β-catenin and matrilysin expression with the progression of colorectal carcinoma. METHODS: High sensitive SP immunohistochemical method was used to detect the proteins of β-catenin and matrilysin in clinical specimens. RESULTS: In some cases of adenoma, early colorectal cancer, advanced colorectal cancer, abnormal β-catenin expression with loss of membranous immunoreactivity or cytoplasmic or nuclear staining was observed and the cytoplasm expression of matrilysin was demonstrated. The abnormal β-catenin expression decreased from normal muscosa, aberrant crypt foci, adenoma, early-colorectal-cancer-to-advanced-colorectal cancer (P<0.05, respectively), and the positive expression of matrlysin gradually increased. The membranous expression rates of β-catenin gradually decreased with tumor cell dedifferentiation (P<0.05). Cytoplasmic or nuclear expression of β-catenin or matrilysin expression was not significantly associated with histological differentiation (P>0.05). The abnormal β-catenin expression and matrilysin positive rate in early colorectal cancer were significantly associated with the depth of invasion and metastasis (P<0.05, respectively). In early colorectal cancer, the matrilysin expression was not correlated with the membranous expression of β-catenin (P>0.05), while it was positively correlation with the cytoplasmic or nuclear expression of β-catenin (P<0.05, respectively). CONCLUSION: β-catenin and matrilysin may play an important role in the carcinogenesis, infiltration and metastasis of colorectal cancer.