鸡胚发育过程中垂体促乳素的表达及其细胞的发育

Developing changes of the pituitary lactotropes in the developing chicken embryo

利用免疫荧光标记方法研究了鸡胚发育过程中垂体促乳素 (PRL)细胞的发生。结果表明 ,在鸡胚发育的第 14 5天可观察到有少量PRL免疫荧光阳性细胞散在分布于腺垂体前叶。随着胚龄的增加 ,垂体PRL细胞的体积逐渐增大 ,细胞直径从第 14 5天的 6 83μm增加到第 2 0 5天的 11 2 4 μm ,胞核直径从 4 2 7μm增加到 5 99μm。PRL细胞占腺垂体细胞总数的百分比在胚胎发育的第 14 5天为 0 86 % ,第 2 0 5天增加到 18 85 % ,并且分布于整个腺垂体前叶 ,细胞多呈索状排列或团状分布。研究结果表明 ,鸡胚垂体催乳素细胞的增殖。

In the present study, the ontogeny of pituitary lactotropes and the changes of cellular morphology, distribution and number containing PRL were detected in the developing chicken embryos from day 3 5 (E) to E20 5 of incubation by using immunofluorescenchemistry. The results indicated that prolactin (PRL) positive cells were first detected at E14 5 of incubation in cephalic lobe of the anterior pituitary, and this distributing pattern persisted in the duration of this study. In addition, the diameters of PRL positive cells and the nuclear at E14 5 were 6 83?μm and 4 27?μm, which increased to 11 24?μm and 5 99?μm by E20.5 respectively. Most of them arranged in grapes. Cell counting results showed that the percentage of PRL positive cell accounting for the total pituitary cells was about 0 86 % at E14 5 of incubation, after which this percentage significantly increased and rose up to 18 85 % by E20 5. These results suggest the proliferation, differentiation and the functional initiation of pituitary lactotrope occur in the late stage of the developing chicken embryos, but the related mechanisms need to be elucidated further.