烟青虫精母细胞减数分裂及单倍体核型研究

Spermatocytes monoploid and karyotype of Helicoverpa assulta

采用压片法HSG显带技术 ,对烟青虫HelicoverpaassultaG櫣ｅｎｅｅ精母细胞减数分裂粗线期进行染色体组型研究 ,描述减数分裂各时期特征 ,分析其染色体数目频率分布与核型特征。烟青虫雄虫部分染色体为弥散型着丝粒染色体 ,另有部分染色体着丝点清晰可见 ,各染色体长从 1 2 7～ 5 0 1μm不等 ;单倍体染色体数n =31,其中常染色体 30条 ,性染色体 1条 ;按Lima De Faria标准 ,烟青虫单倍体中含 5条中型染色体和 2 6条小型染色体 ;分裂相中染色体数目为 31条者比例超过 5 0 % 。

With HSG banding technique, we analyzed the karyotype of Helicoverpa assulta Güenee (Lepidoptera: Noctuidae) during meiosis pachytene.The results showed that the number of haploid monoploid chromosomes of H. assulta was n =31.There were 30 autosomes and one of sex chromosome in 31 chromosome of themale bollworm haploid, the most common number in Lepidopteran species. Part of chromosome was holocentromere. The chromosome length of H. assulta ranged from 1 27?μm to 5.01?μm. Based on the standard of Lima De Faria, there were also 5 middle sized and 26 small sized chromosomes among all the haploid. More than half of H. assulta had 31 chromosomes at their split phaseshowing that the chromosome polymorphism of H. assulta was much notable.