培养条件对木霉菌合成木聚糖酶的影响

Influence of culture condition on the synthesis of xylanase from Trichoderma.

能促进双歧杆菌增殖的功能性低聚糖的研究开发具有重大的意义 ,其中最有效的木低聚糖可从木质纤维的有控酶降解反应获得 .为此 ,可以采用培养木霉菌 ,使其在最佳条件下比较专一性的合成木聚糖酶 ,这种最佳条件包括培养基成分的探索和培养条件的控制两方面 .期望用这种木聚糖酶降解木质纤维原料时 ,能尽可能多的得到木低聚糖 ,而较少出现木单糖 .该文就木霉合成木聚糖酶过程中的培养时间、培养温度、通气条件、pH值等 ,对木聚糖酶活性的影响进行了研究 .结果表明 ,木聚糖酶活性在培养 84h时达到最大值 ;当将温度控制在 30℃ ,摇床转速为 180r min时 ,木聚糖酶活性最高 ,达到 2 0IU mL ;酶合成的最适pH值为 5 0 .

Oligosaccharide were able to proliferate effectively, and among them xylo-oligosaccharide was the best one. Xylo-oligosaccharide can be synthesized by controllable enzymic-degradation of wood fiber. Trichoderma was cultured and xylanase was synthesized under optimal conditions including choosing the culture medium ingredients and controlling the culture condition. In the degradation of wood fiber by xylanase, more xylo-oligosaccharide and less xylose were hoped. There are many factors affecting the characteristics of xylanase produced by Trichoderma. This paper studied the effects of culture time, culture temperature, quantity of oxygen, and pH on the activity of xylanase. The results show that the optimal culture time is 84 h, the optimal culture temperature is 30°C, the optimal rotating speed of reactor is 180 r/min, and the optimal pH is 5.0. Under these conditions, the activity of xylanase is 20 lU/mL.