鸽源Ⅰ型禽副黏病毒HN基因序列测定和分析

Sequencing and Analysis on the HN Gene of Avian paramyxovirus type I Isolated from Pigeon

以分离自云南省的一株鸽源禽I型副黏病毒YN-P1株的基因组RNA为模板,通过RT-PCR的方法扩增出HN基因片段,然后将其克隆至T载体中。经PCR鉴定后,对阳性克隆进行核苷酸序列测定。测序后拼接得出HN基因的全序列。测序结果显示,YNP1毒株HN基因为1 716 nt。通过与参考毒株比较HN基因序列,发现所研究毒株YN-P1与TW95核苷酸序列同源性最高为88.3％;与ZJ1／Go氨基酸序列同源性最高为91.6％。

Virion RNA was abstracted from Avian paramyxovirus type I YN-P1 strain (isolated from pigeon in Yunnan Province) and used as a template. The fragment of hemagglutinin-neuriminidase(HN) gene of the isolate was amplified by RT-PCR and ligated with T vector. Positive clones of HN gene were identified by PCR and then sequenced. The complete nucleotide sequence of HN gene was obtained finally. The sequencing result showed that HN gene length of the YN-P1 strain 1 716 nt. The results of sequence analysis indicated that YN P1 strain displays the highest nucleotide homology (88. 3%) in comparision with reference strain TW95 and highest amino acid homology(91. 6%) in comparision with strain ZJ1/Go.