致病性鹅源新城疫病毒对鸡胚成纤维细胞的致病变特性

Cytopathic Alterations of Chicken Embryo Fibroblasts Induced by Newcastle disease virus of Goose Origin

选择4株鹅源新城疫病毒(NDV)接种鸡胚成纤维细胞(CEF)培养物,研究鹅源NDV对CEF的致病变特性,并与鸡源及鸽源NDV进行比较。结果显示,鹅源NDV接种次代CEF后24h开始有病变产生,表现为少数细胞变圆、皱缩,折光性增强,随后病变缓慢进展,感染细胞形成大量合胞体,到84-144 h时,CEF单层彻底破坏;鸡源和鸽源NDV接种CEF后也在24 h左右开始有病变产生,最初病变与鹅源毒株导致的病变相似,但病变进展迅速,细胞显著裂解并导致单层的严重破坏,60-84 h时细胞单层即彻底破坏。对病毒接种后不同时间段培养物上清血凝(HA)效价的测定结果表明,鹅源NDV接种后84-120h培养物上清HA效价达到高峰,为5-8 log2,鸡源和鸽源NDV接种后60-84 h HA效价即可达到高峰,但其最高效价只有4 log2。

The cytopathic effects on chicken embryo fibroblasts (CEFs), induced by Newcastle disease virus (NDV) derived from the novel disease outbreaks in geese during 1997 - 2000, were compared with those induced by chicken and pigeon isolates. When the CEFs were inoculated with goose isolates, cell abnormalities could be seen as early as 24 h PI, characterized by occurrence of some rounded,refractile and dark appearing cells. Then the lesions became more severe gradually, with a large number of syncytial cells appeared. The CEF monolayers were destroyed completely at 84-144 h PI, and the HA titers in the culture supernatants could get as high as 5-8 log2. When infected with ND viruses of chicken or pigeon origin, the earliest cell lesions were observed at 24 h PI, too. But it appeared that the alterations developed more rapidly in this occasion. The cells became rounded and refractile and necrosized, leaving empty areas (holes) in the cell sheet. At 60-84 h PI, the monolayers destroyed, with HA titers of 3-4 log2 in the culture supernatant. The syncytial cells contained far less nuclei than those formed after inoculation of goose isolates.