La蛋白与HBVRNA相互作用关系的研究进展

Study on interaction between human La protein with hepatitis B virus RNA

新近研究发现 ,人类 L a蛋白 (hum an L a protein,h L a)是一种与 HBV RNA转录调节相关的因子。L a蛋白可能结合在 HBV RNAs的茎环结构上 ,具有保护乙肝病毒 (hepatitis B virus,HBV) RNA,促进其翻译启动的作用 ,可能是 HBV RNA的稳定因子。当 L a蛋白发生突变后将无法与 HBV RNA结合 ,丧失保护 HBV RNA的功能 ,使其受到酶的降解 ,无法转录翻译 ,病毒的复制受阻 ,从而抑制 HBV感染。本文综述了 L a蛋白、HBV RNA及核酶 (nuclear RNases)三者间相互作用关系的最新研究成果 ,从分子水平上探讨了导致 HBV RNA降解的可能机制 ,推测了野生型和突变型 L a蛋白对乙型肝炎病毒

The human La protein is recently identified as a host factor potentially involved in the post-transcriptional regulation of hepatitis B virus (HBV) RNA. The La binding site is mapped to a predicted stem-loop structure within a region shared by all HBV RNAs, and it is known that human La protein protected HBV RNA against Rnase-mediated degradation. HLa mutants lost the ability of binding and protecting HBV RNA, which make it easy for HBV RNA to degrade and the virus replication to terminate. This review summarizes the latest investigation about the interaction of La protein,HBV RNA and Nuclear RNases, and discusses the possible mechanisms of HBV RNA degradation, the effect of La protein and its mutants on the translation initiation of HBV RNA, and the replication of the virus.