新生大鼠磷脂酶C-γ1mRNA的表达变化

Phospholipase C-γ1 dynamic mRNA expression patterns in rats during early postnatal period

目的探讨新生大鼠早期各主要脏器磷脂酶C-γ1(PLCγ1,基因为PLCG1)的mRNA表达状况。方法应用半定量逆转录聚合酶链反应(RT-PCR)技术分析大鼠出生后1、3、5、7 d及2、3、5周等7个时期肝、肺、肾和脑等4种脏器共28例样本的PLCG1的表达变化,以看家基因磷酸甘油脱氢酶(GAPDH)为内参照,同步逆转录PLCG1特异性片段和内参片段,以二者积分吸光度比值作为PLCG1 mRNA的相对表达量。结果肝、肺、肾和脑4种脏器组织PLCG1在出生后早期的不同时期均有较强表达,并且各时期之间的表达有显著性差异(P均<0.01),第1天肝脏组织中PLCG1几乎没有表达,第7天脑组织的表达最高;而在同一时期4种不同的组织间PLCG1的表达也有显著性差异(P均<0.01)。结论同种脏器组织在不同的发育时期及在同一时期不同脏器组织之间PLCG1的差异性表达提示,PLCG1可能参与了大鼠早期生长发育过程中细胞的增殖与分化。

Objective To investigate the expression of phospholipase C-γ1 (PLCG1) mRNA in rats during early postnatal period. Methods Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of PLCG1 mRNA in 28 samples extracted from the liver, lung, kidney and brain of rats aged 1, 3, 5, 7 days, and 2, 3, 5 weeks. Specific PLCG1 product and GAPDH product as the internal control were both amplified by RT-PCR, and the ratio of their integral optical density was calculated to estimate the relative mRNA expression of PLCG1. Results PLCG1 was expressed in rat liver, lung, kidney and brain at the 7 postnatal time points, and the expression varied significantly with time and between the different organs (P<0.01), virtually undetectable in the liver on postnatal day 1 and reaching the highest level in the brain tissues on postnatal day 7. Conclusion The differences in PLCG1 expression in various organs and development periods suggest that PLCG1 is involved in cell proliferation and differentiation during the early development of rats.