星形胶质细胞条件培养液对tbOOH致PC12细胞凋亡的抑制作用

Effect of astrocyte-conditioned medium on tert-butyl hydroperoxide-induced apoptosis of PC12 cells

目的探讨星形胶质细胞条件培养液对活性氧叔丁基脂氢过氧化物tbOOH诱导的PC12细胞凋亡的影响。方法以分离、纯化的SD大鼠大脑皮层星形胶质细胞制备的条件培养液培养tbOOH应激的PC12细胞,采用荧光显微镜和透射电镜形态观察细胞凋亡;用流式细胞术测定细胞凋亡率的变化;用巴比妥酸法评估细胞内丙二醛含量的变化。结果tbOOH可诱导PC12细胞凋亡,而星形胶质细胞条件培养液可降低其凋亡;同时,巴比妥酸法显示星形胶质细胞条件培养液可显著性降低PC12细胞丙二醛含量(P<0.05)。结论星形胶质细胞条件培养液有提高PC12细胞抗氧化能力,可抑制活性氧tbOOH诱导的神经细胞凋亡。

Objective To investigate the effect of astrocyte-conditioned medium (ACM) on PC12 cell apoptosis induced by reactive oxygen species tert-butyl hydroperoxide (tbOOH). Methods PC12 cells attacked by tbOOH were cultured with Sprague Dawley rat cerebral cortex ACM, and the cell apoptosis was observed by fluorescent microscopy and transmission electron microscopy. The cell apoptosis rate was evaluated by flow cytometry and malondialdehyde (MDA) concentration measured using thiobarbituric acid (TBA)-reacting substances (TRABS). Result TbOOH induced PC12 cell apoptosis, and ACM significantly decreased the apoptosis rate and MDA concentration in tbOOH-treated cells. Conclusion ACM may increase the anti-oxidation capacity of PC12 cells and inhibit cell apoptosis induced by tbOOH.