钩吻总生物碱中钩吻素子的提取与分离

Extraction and separation of koumine from Gelsemium alkaloids

目的研究钩吻中总生物碱的提取、总量测定方法及其中主要成分钩吻素子的分离、鉴定方法。方法采用加热回流提取与氯仿萃取相结合的方法提取总生物碱;采用碱性硅胶柱层析、梯度洗脱法分离钩吻素子并用薄层层析法和紫外分光光度法定性鉴别。结果从钩吻中分离得到了无色棱柱状钩吻素子晶体。结论本实验所采用的提取钩吻总生物碱及分离钩吻素子的方法行之有效。

Objective To establish a method for isolating and identifying koumine. Methods Koumine was first extracted from Gelsemium alkaloids by using chloroform, which was further separated and purified by column chromatograph, and identified by thin-layer chromatography. Results and Conclusion Koumine crystals were isolated and purified from Gelsemium alkaloids effectively.