紫河车体外对黑素细胞增殖及酪氨酸酶活性的影响

Hominis placenta promotes melanocyte proliferation,melanin synthesis and tyrosinase activity in vitro

目的观察中药紫河车对黑素细胞和酪氨酸酶的影响。方法采用MTT法测定黑素细胞增殖情况,Hunt法测定黑素合成,以蘑菇酪氨酸酶多巴速率氧化法测定酪氨酸酶活性。结果(1)紫河车对黑素细胞具有增殖作用,其最佳增殖浓度为0.1g/L(P<0.01);(2)紫河车可增强黑素合成能力,其最佳增强能力为0.1g/L(P<0.01);(3)紫河车对酪氨酸酶亦具有激活作用,其最佳激活浓度为1g/L(P<0.01)。结论紫河车能激活黑素细胞增殖、黑素合成、酪氨酸酶活性。

Objective To investigate the effects of Hominis placenta on melanocytes and tyrosinase activity in vitro. Methods MTT assay was used to assess the proliferation of melanocytes treated with Hominis placenta. Hunt method was employed to determine melanin synthesis and the oxidation rate of DL-dopa to evaluate the tyrosinase activity. Results Hominis placenta promoted melanocyte proliferation (P<0.01) and significantly increased the cell number (P<0.01), melanin synthesis (P<0.01), with the optimal concentration of 0.1g/L (P<0.01), and also enahnced tryosinase activity (P<0.01) at the optimal concentration of 1 g/L (P<0.01) . Conclusion Hominis placenta promotes proliferation, melanin synthesis and tyrosinase activity of the melanocytes.