我国三省区细粒棘球绦虫基因的变异分析

Analysis of genetic variation of Echinococcus granulosus collected from three provinces in China

对从青海省、甘肃省和新疆维吾尔自治区采集的 2 4株细粒棘球蚴 ,用线粒体DNA的CO1基因和ND1基因结合rDNA的ITS2测序 ,调查了不同地区分离株基因型的变异情况。结果显示 ,所有分离株均为普通羊株 (G1基因型 ) ;CO1基因序列的变异率为 0～ 0 .8% ,ND1基因的变异率为 0 .1%～ 0 .7% ;青海分离株ITS2序列的变异率为 0 .4 %～ 3.1% ;2株青海省西宁市和 1株甘肃省武威市分离株分别在CO1基因和ND1基因序列不同位点发生的 1处核苷酸非同义替换 ,导致 1个编码的氨基酸替代。研究表明 ,我国上述 3省、区部分区域存在的细粒棘球蚴属于同一株 (G1基因型 )。

Twenty-four Echinococcus granulosus isolates, which were collected from Qinghai Province, Gansu Province and Xinjiang Region in China, were examined with CO1, ND1 and ITS2 sequencing to (investigate) genotype of the isolates. The results were as follows. All the Echinococcus granulosus isolates were identified to be the common sheep strain (G1 genotype). When nucleotide sequences of both CO1 gene and ND1 gene were compared, variation percentage of 0-0.8% and 0.1%-0.7% existed respectively. Variation percentage of ITS2 of the isolates from Qinghai Province was 0.4%-3.1%. Two isolates from Qinghai Province and one isolate from Gansu Province had non-synonymous substitations at the (different) positions of CO1 gene and NO1 gene sequences, which resulted in one amino acid replacement substitution. The study indicated that one strain (G1 genotype) existed in parts of Qinghai, Gansu and (Xinjiang.)