双相支架负载软骨细胞修复兔关节软骨缺损

Rabbit articular cartilage defect treated with cultured chondrocytes embedded in two-phase scaffold

目的 研究自固化磷酸钙 /纤维蛋白凝胶 (CPC/FG)双相支架负载软骨细胞修复兔关节软骨缺损的可行性和有效性。方法 将分离培养的第 3代软骨细胞包埋在CPC/FG双相支架的FG中 ,体外培养 1周后 ,将软骨细胞 -支架复合体移植修复兔膝关节股骨髁的软骨缺损 ( 4mm ,深 3 5mm ,达软骨下骨质 )。然后对软骨缺损的修复情况进行大体、光镜和电镜观察。同时对移植后第 12周的修复软骨进行胶原含量测定 ,并与正常的关节软骨细胞胶原含量进行比较。结果 移植的软骨细胞能在双相支架上良好地生长 ,软骨缺损以透明软骨的形式被修复 ,而对照组为纤维组织修复。多孔自固化磷酸钙在软骨修复过程中能起软骨下骨的临时替代作用。胶原含量测定显示 :移植术后 12周的修复软骨胶原含量为 (4 3 2 5± 0 85 ) % ;正常的关节软骨胶原含量为 (5 5 6 9± 0 76 ) % ,两者差异有显著性 (P <0 0 1)。结论 CPC/FG双相支架负载软骨细胞能以透明软骨的形式修复兔关节软骨缺损。新环境中移植的软骨细胞生长的不适应和FG降解过快 。

Objective To evaluate the effectiveness and feasibility of the cultured articular cartilage chondrocytes embedded in CPC/FG two-phase scaffolds to repair rabbit articular cartilage defects based on tissue engineering. Methods The third passaged chondrocytes were embedded in the two-phase scaffolds' FG,After 1 week of cultured in vitro, the complexes of chondrocytes-scaffold were transplanted to repair the articular cartilage defects of femoral condyle of rabbit knees (the defects were 4 mm in diameter, 3.5 mm in depth, extending down to the subchondral bone). Healing of defects was assessed by gross examination, light microscope and TEM. In addition, the collagen contents of the healing cartilages after 12 weeks of transplantation and of the normal cartilages were detected and compared. Results The transplanted chondrocytes grew well on the scaffold, and the articular cartilage defects were repaired by hyaline cartilage. In the no chondrocytes transplantation groups, the defects were repaired only by fiber tissue. In addition, the porous CPC was temporary substitute of subchondral bone in the period of repairing. The collagen content of healing cartilage after 12 weeks of transplantation was (43.25±0.85)%, and the content of normal cartilage was (55.69±0.76)%,The difference between the two groups was statistically significant (P<0.01). Conclusion The two-phase scaffold of CPC/FG embedded chondrocytes can repair the cartilage defects of femoral condyle of rabbit knee successfully in the mode of hyaline cartilage. The too fast degradation of FG and the unadaptation of chondroytes in new situation maybe the reasons of the difference of collagen content between repaired cartilage and normal cartilage which was statistically significant.