白念珠菌菌丝相随机扩增多态性DNA分析

Analysis on Hyphal form of Candida Albicans by Randomly Amplified Polymorphic DNA

目的 以白念珠菌菌丝相为研究对象 ,比较对氟康唑敏感程度不同的白念珠菌之间随机扩增多态性DNA(RAPD)图谱的差异。方法 用随机引物 5′ TCACCACGGT 3′对同一亲本来源的 16株白念珠菌菌丝相基因组DNA进行扩增 ,扩增产物进行变性聚丙烯酰胺凝胶电泳并拍照。结果 菌株CA 1～CA 13的电泳带型一致 ,均有 6条电泳带 ;CA 14～CA 17的电泳带型一致 ,均有 7条电泳带 ;CA 14～CA 17比CA 1～CA 13多 1条约 45 0bp的电泳带。

Objective To compare the randomly amplified polymorphic DNA (RAPD) map difference among susceptible,susceptible dose-dependent and resistant hyphal form of Candida albicans strains with fluconazole.Methods DNAs extracted from hyphal form of C. albicans were amplified by PCR with the same arbitrary sequence decamer primer 5′-TCACCACGGT-3′,and the PCR products were separated by denaturing polyacrylamine gel electrophoresis (PAGE) and photoed.Results The electrophoresis bands of CA-1-CA-13 were identical,they all had 6 bands;The electrophoresis bands of CA-14-CA-17 were identical,they all had 7 bands;a band of about 450bp was specific to substains CA-14-CA-17.Conclusion There was difference on RAPD map between resistant and susceptible substrains hyphal form of C.albican.