IL-12对SLE患者PBMC分泌IL-10、IFN-γ的影响

Effect of Interleukin-12 on IL-10 and IFN-γ Production by PBMC in Patients Vitro

目的 了解白细胞介素 12 (IL 12 )对SLE患者外周血单一核细胞 (PBMC)分泌白细胞介素 10 (IL 10 )、γ 干扰素 (IFN γ)的影响。方法 在培养体系中加入或不加入IL 12 ,观察IL 12对SLE患者及正常对照PBMC分泌IL 10 ,IFN γ的水平变化。结果 IL 12可使SLE患者PBMC分泌IL 10水平降低而使IFN γ水平升高。结论 在体外 ,加入适当的IL

Objective To explore the IL-10 and IFN-γ Production in Supernatant of Systemic Lupus Erythematosus if Interleukin-12 added in vitro. Methods Using ELISA to measure the IL-10 and IFNγ levels in supernatant of PBMC from SLE patients and normal controls respectively, when IL-12 was added or not to the culture system.Results IL-12 up-regulated the IFN-γ level and down-regulated the IL-10 level in supernatant of SLE PBMC.Conclusion In vitro, IL-12 can rectify some abnormal cytokine phonotype in SLE.