摘要
目的 :探讨乙型肝炎病毒慢性感染 C基因启动子 (BCP)变异对病毒复制水平及肝功能损害程度的影响。方法 :采用PCR微板核酸杂交结合 EL ISA检测显示技术 ,检测 74例乙型肝炎病毒慢性感染者 BCP区核苷酸 (nt) 176 2碱基 A→ T和176 4碱基 G→ A联合突变。结果 :在 74例乙型肝炎病毒慢性感染者中检出 BCP区 T176 2 A 176 4突变 2 4例 (32 .4 % ) ,BCP变异阳性组的 HBVDNA水平 (10 8.2 992± 0 .86 6 5拷贝 / m l)显著高于 BCP变异阴性组的水平 (10 7.1 737± 1 .1 539拷贝 / ml) (P <0 .0 0 1) ;BCP变异组的肝功能损害程度较非变异组明显。结论 :BCP变异可引起 HBV致病力增强 ,复制水平提高 。
Objective:To investigate relationship between HBV c gene promoter (BCP) mutation and serum HBV DNA contents and liver lesion in patients with chronic hepatitis B virus infection.Methods:Combining polymerase chain reaction with ELISA, mutants (nt1762A→T and 1764G→A) of HBV BCP in 74 patients with chronic hepatitis B virus infection were studied.Result:The T1762 and A1764 mutants in HBV BCP region was found in 24 (24/74) patients.The quantity of HBVDNA in mutant group (10 8.299 2±0.866 5 copy/ml) was significantly higher than that in non-mutant group (10 7.173 7±1.153 9 copy/ml) (P<0.001). Serum TBiL ALB ALT AST/ALT levels showed significant differences between BCP mutant group and non-mutant group.Conclusion:The T1762 A1764 mutants in HBV BCP region may lead to the replication of HBVDNA.BCP mutant patients suffer from a more severe liver lesion.
出处
《广西医科大学学报》
CAS
2004年第4期486-488,共3页
Journal of Guangxi Medical University
基金
广西自然科学基金项目 (桂科自 0 3 3 90 5 0 )
