黄斑裂孔性视网膜脱离患者外周血T细胞亚群的研究

ANALYSIS OF PERIPHERAL BLOOD T LYMPHOCYTE SUBSETS IN PATIENTS OF RETINAL DETACHMENT WITH MACULAR HOLE

目的 :探讨黄斑裂孔性视网膜脱离患者外周血 T淋巴细胞亚群的变化 ,了解该病患者机体免疫平衡状态。方法 :使用单克隆抗体碱性磷酸酶抗碱性磷酸酶法 (APAAP)检测 4 0例黄斑裂孔性视网膜脱离患者和 4 0例正常人外周血 T淋巴细胞亚群 ,并作对照分析。结果 :黄斑裂孔性视网膜脱离组的 CD3+ 、CD4 + 细胞与细胞总数的比值较正常对照组低 (P <0 .0 5 ) ,CD8+细胞的比值高于对照组 (P <0 .0 5 ) ,CD4 + / CD8+的比值比正常对照组低 (P <0 .0 1)。结论

Objective:To evaluate the cell-mediated immunity in cases of retinal detachment with macular hole by measuring peripheral blood T lymphocyte subsets with monoclonal antibody techinique.Methods:40 cases of retinal detachment with macular hole and 40 healthy persons were enrolled and their peripheral blood T lymphocyte subsets were mearsured with Alkaline Phosphatase Anti-Alkaline Phosphatase (APAAP).Result:Ratio of CD 3 +,CD 4 + from retinal detachment group is lower than those from healthy group (P<0.05), when ratio of CD 8 + is higher than the healthy ones (P<0.05), and CD 4 +/ CD 8 + is lower when compared to the healthy group (P<0.01).Conclusion:Human's cell-mediated immunity has disorder when there is retinal detachment with macular hole onset.