双苯氟嗪小鼠急性毒性及体内致突变毒性评价

EVALUATION OF ACUTE TOXICITY AND MUTAGENIC TOXICITY OF DIPFLUZINE IN VIVO

目的考察双苯氟嗪 (dipfluzine)对小鼠的急性毒性及体内致突变毒性。 方法小鼠灌胃给予双苯氟嗪 ,计算LD50 ;微核实验设 6组 :空白对照组 (水 )、溶剂对照组 (5g/L羧甲基纤维素钠 )、阳性对照组 (环磷酰胺 )、和双苯氟嗪 3个剂量组 (2 .8、1 .4和 0 .7g/kg) ,于给药 2 4h后取骨髓 ,计数各组骨髓细胞微核率 ;骨髓细胞染色体畸变实验 :小鼠给药与分组同微核试验 ,分别于给药 1 2、2 4、4 8h后取骨髓进行染色体畸变分析。结果小鼠灌胃给予双苯氟嗪LD50 为 (5 6 81± 6 5 0 )mg/kg ;剂量分别为 2 .8、1 .4和 0 .7g/kg时 ,微核实验和骨髓细胞染色体畸变实验中 ,各给药组骨髓细胞微核率和染色体畸变率与空白对照组比较均无显著性差异 (P均 >0 .0 5 ) ,也无剂量反应关系 ,实验结果均为阴性。结论双苯氟嗪在小鼠体内无致突变作用。

ObjectiveTo investigate the acute toxicity and mutagenic toxicity of dipfluzine in vivo. MethodsThe mice were administered orally with dipfluzine and LD 50 was obtained. Micronucleus (MN) test: the experiment consisted of 6 groups, a control group(water), a solvent group(5 g/L carboxymethylcellulose sodium), a positive control group(cyclophosphamid) and three dipfluzine groups( 2.8 , 1.4 and 0.7 g/kg). The bone marrow of mice was taken at 24 h after giving drug and the MN rates were calculated. Mouse bone marrow chromosome aberration(CA) test: the groups and treating ways were same as MN test. The bone marrow of mice was taken at 12, 24, 48 h after giving drug and the CA was analysed. ResultsLD 50 was ( 5 681 ±650) mg/kg for the mice administered orally with dipfluzine. The MN rates and CA rates of bone marrow cells in dipfluzine groups had no significant difference with those in control group. Also, there was no dose dependence both in MN test and mouse bone marrow CA test between dipfluzine orally taken 2.8 , 1.4 and 0.7 g/kg. ConclusionDipfluzine has no mutagenic toxicity in vivo.