摘要
目的探讨葛根素对糖尿病大鼠肾功能、组织结构以及过氧亚硝基阴离子 (peroxynitrite ,ONOO-)特异性标志物硝基酪氨酸 (nitrotyrosine ,NT)表达的影响。方法一次性腹腔注射链脲佐菌素 6 5mg/kg复制大鼠糖尿病模型 ,用药组每日腹腔注射葛根素注射液 ,共 1 6周。收集 2 4h尿液测定尿蛋白、尿肌酐 ;腹主动脉取血分离血清测定血糖、血肌酐、血尿素氮。光镜下观察肾组织病理形态学改变 ,采用免疫组织化学技术观察肾组织中NT的表达。结果与对照组比较 ,糖尿病组出现肾功能下降 ,肾小球及肾小管肥大等异常表现 ,同时NT表达明显增加 ;应用葛根素后肾功能及肾组织病理改变有明显改善 ,且NT表达明显下降。结论葛根素可降低肾组织NT表达 ,从而减轻肾组织氧化损伤 ,这可能是其减轻糖尿病所致大鼠肾脏功能结构异常的作用机制之一。
ObjectiveTo investigate the effects of puerarin on renal structure, function and the expression of nitrotyrosine(NT) as the specific symbol of peroxynitrite (ONOO -) in diabetic rats. MethodsDiabetes mellitus models were established in rats by injecting streptozotoein (65 mg/kg) once intraperitoneally. The drug administration groups had daily injections of puerarin intraperitoneally for 16 weeks after induction of diabetes mellitus. The urine was collected for 24 hours as sample of urine protein and urine creatinine determination. Blood was sampled from the abdominal aorta, and serum was isolated for determination of blood glucose, blood creatinine and blood urea nitrogen. Pathological changes of the renal tissues were observed under light microscope. NT expression in renal tissues was detected by immunohistochemical technique. ResultsCompared with control group, abnormalities concerning kindey function as well as morphological changes such as hypertrophy of glomerulus and renal tubule were observed in diabetic rats, and NT expression was increased in diabetic kidney. Puerarin administration improved the kidney function and its pathological changes, and resulted in lower expression on NT in the kidney compared with those in diabetes mellitus group. ConclusionPuerarin brings down NT expression in the renal tissues and alleviates the oxidative injury to the kidney, which may act as a mechanism in alleviation of renal function and structure abnormities in rats by diabetes mellitus.
出处
《河北医科大学学报》
CAS
2004年第5期263-265,279,共4页
Journal of Hebei Medical University
