尼美舒利对人结肠癌细胞生长的抑制作用及对E-钙粘蛋白表达的影响

Effects of nimesulide on the proliferation and the expression of E-cadherin of human colonic carcinoma cell lines

目的 探讨尼美舒利对体外培养的结肠癌细胞生长及E cad表达的作用。方法 以人结肠癌细胞株HT 2 9,HCT 116为研究对象 ,体外药物敏感实验 (MTT)法检测尼美舒利对肿瘤细胞的增殖抑制效应 ;应用免疫组化S P方法检测尼美舒利作用前后细胞E cad表达的变化。结果 尼美舒利对人结肠癌细胞HT 2 9、HCT 116生长的抑制作用呈时间、剂量依赖性方式 ,对HT 2 9细胞抑制效果强于HCT 116细胞。尼美舒利上调HT 2 9细胞E cad表达 ,而对HCT 116细胞E cad的表达无显著差异。结论 尼美舒利可明显抑制COX 2表达阳性的结肠癌细胞HT 2 9生长 ,同时提示尼美舒利可能通过抑制COX 2酶活性而上调肿瘤细胞E cad的表达 。

Objective To evaluate the potential roles of nimesulide, a nonsteroidal anti inflammatory drug (NSAID), on the proliferation and the expression of E cadherin of human colon adenocarcinoma cell lines. Methods The human colon cancer cell lines HT 29 and HCT 116 were employed in the study. After treatment with nimesulide, the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay, and the expression of E cadherin in the cells was detected by immunohistochemical streptavidin/peroxidase method. Results Nimesulide inhibited the proliferation of HT 29 and HCT 116 cells in time and dose dependent manners. The inhibitory effect on HT 29 cells was stronger than that on HCT 116 cells. Nimesulide up regulated the E cadherin expression of HT 29 cells, whereas the expression level of HCT 116 cells remained unchanged. Conclusion Nimesulide can obviously inhibit the growth of colon cancer cells HT 29 overexpressing cyclooxygense 2 protein, suggesting that nimesulide may up regulate the expression of E cadherin through the inhibition of COX 2 activity, resulting in decrease of the invasion and metastatic ability of tumor cells.