摘要
利用ISSR技术对 1 2个莲藕品种进行了DNA多态性分析。从 6 5个随机引物中筛选出 7个引物扩增基因组DNA ,共获得 91条带 ,其中 75条为多态性标记 ,每个引物平均提供 1 3个标记信息。由UPMGA方法得到的聚类分析结果表明了1 2个品种间的亲缘关系 。
The ISSR analysis was conducted in 12 lotus. From 65 random primers, 7 primers were selected and then amplified the 12 genomic DNAs. 91 RAPD fragments were generated, 75 of them were polymorphic marks. Every primer offered 13 markers on average. The molecular phylogenetic tree conducted by UPMGA method and showed the genetic relationship among 12 lotus, which was better identical with pedigree analysis.
出处
《氨基酸和生物资源》
CAS
2004年第3期20-22,共3页
Amino Acids & Biotic Resources
基金
国家科技攻关项目 ( 2 0 0 2BA5 46C)
湖北省科技攻关重大项目 ( 2 0 0 2AA2 0 5A )
武汉市科技攻关重点项目( 2 0 0 2 2 0 0 5 13 2 )
关键词
莲藕
遗传
多态性
亲缘关系
品种
ISSR
lotus
Inter Simple Sequence Repeats
polymorphism
genetic relationship
